01 | 亲子鉴定 Paternity Testing |
02 | 个人识别 Personal Identification |
03 | 细胞鉴定 Cell Lines Authorization |
04 | 同胞鉴定 Sibling Testing |
05 | 基因档案 Gene Archive |
06 | 种属鉴定 Species Identification |
07 | 祖孙鉴定 Kinship Testing |
应用于生物医学研究领域的哺乳动物细胞被错误鉴定和交叉污染的问题,一直是普遍存在的一个突出问题。据统计,国外实验室有20%左右的细胞株被错误鉴定和交叉污染,NIH和ATCC近两年都对此发出呼吁,要求研究者对细胞进行鉴定。一些权威杂志在作者投稿时就要求提供细胞株的STR基因型鉴定证明。 北京大学 清华大学 西安交通大学 上海交通大学 [1]. Yu J, Xu QG, Wang ZG, Yang Y, Zhang L, Ma JZ, Sun SH, Yang F, Zhou WP. Circular RNA cSMARCA5 inhibits growth and metastasis in hepatocellular carcinoma. J Hepatol. 2018 Jun;68(6):1214-1227. PMID: 29378234 [2]. Zhang N, Zhang H, Liu Y, Su P, Zhang J, Wang X, Sun M, Chen B, Zhao W, Wang L, Wang H, Moran MS, Haffty BG, Yang Q.SREBP1, targeted by miR-18a-5p, modulates epithelial-mesenchymal transition in breast cancer via forming a co-repressor complex with Snail and HDAC1/2. Cell Death Differ. 2018 Jul 9. doi: 10.1038/s41418-018-0158-8. PMID: 29988076 [3]. Du L, Han XG, Tu B, Wang MQ, Qiao H, Zhang SH, Fan QM, Tang TT. CXCR1/Akt signaling activation induced by mesenchymal stem cell-derived IL-8 promotes osteosarcoma cell anoikis resistance and pulmonary metastasis. Cell Death Dis. 2018 Jun 18;9(7):714. PMID: 29915309 [4]. Zhou P, Qin J, Li Y, Li G, Wang Y, Zhang N, Chen P, Li C. Combination therapy of PKCζ and COX-2 inhibitors synergistically suppress melanoma metastasis. J Exp Clin Cancer Res. 2017 Sep 2;36(1):115. PMID:28865485 [5]. Wang X, Cheng Y, Zhu Y, Li H, Ge W, Wu X, Zhao K, Yuan J, Li Z, Jiang S, Han Z, Jiang Q, Wu Q, Liu T, Zhang C, Yu M, Hu Y. Epigenetic silencing of ASPP1 confers 5-FU resistance in clear cell renal cell carcinoma by preventing p53 activation. Int J Cancer. 2017 Jun 28. doi: 10.1002/ijc.30852. PMID: 28656647 [6]. Li Y, Xie P, Lu L, Wang J, Diao L, Liu Z, Guo F, He Y, Liu Y, Huang Q, Liang H, Li D, He F. An integrated bioinformatics platform for investigating the human E3 ubiquitin ligase-substrate interaction network. Nat Commun. 2017 Aug 24;8(1):347. doi: 10.1038/s41467-017-00299-9. PMID: 28839186 [7]. Wang C, Li L, Yin Z, Zhang Q, Zhao H, Tao R, Wang S, Hu S, He Y, Wang D, Li C, Zhang S, Xu J, Jiang X, Zhu S, Gao Y. An Indel Polymorphism within pre-miR3131 Confers Risk for Hepatocellular Carcinoma. Carcinogenesis. 2017;38:168-76. PMID:28034876 [8]. Wang X, Yu M, Zhao K, He M, Ge W, Sun Y, Wang Y, Sun H, Hu Y. Upregulation of MiR-205 under hypoxia promotes epithelial-mesenchymal transition by targeting ASPP2. Cell Death Dis. 2016;7(12):e2517. PMID:27929537 [9]. Li P, Wang Y, Mao X, Jiang Y, Liu J, Li J, Wang J, Wang R, She J, Zhang J, Yang J, Liu Y, Liu P. CRB3 downregulation confers breast cancer stem cell traits through TAZ/β-catenin. Oncogenesis. 2017 Apr 24;6(4):e322. PMID:28436991 [10]. Du J, Shang J, Chen F, Zhang Y, Yin N, Xie T, Zhang H, Yu J, Liu F. A CRISPR/Cas9-Based Screening for Non-Homologous End Joining Inhibitors Reveals Ouabain and Penfluridol as Radiosensitizers. Mol Cancer Ther. 2018 Feb;17(2):419-431. PMID: 28864683 [11]. Chen L, Aleshin AE, Alitongbieke G, Zhou Y, Zhang X, Ye X, Hu M, Ren G, Chen Z, Ma Y, Zhang D, Liu S, Gao W, Cai L, Wu L, Zeng Z, Jiang F, Liu J, Zhou H, Cadwell G, Liddington RC, Su Y, Zhang XK. Modulation of nongenomic activation of PI3K signalling by tetramerization of N-terminally-cleaved RXRα.Nat Commun. 2017 Jul 17;8:16066. PMID:28714476 |